Journal: Pharmaceutics

Article Title: A Novel 2-Methoxyestradiol Derivative: Disrupting Mitosis Inhibiting Cell Motility and Inducing Apoptosis in HeLa Cells In Vitro

doi: 10.3390/pharmaceutics16050622

Figure Lengend Snippet: Compound 4a markedly diminished the invasiveness of cervical cancer cells (HeLa). The anti-invasive potential of the test compound is illustrated by representative images ( B ), and is quantified by the percentage of invading cells in the Boyden chamber containing different concentrations of 4a , using an EMEM medium supplemented with 10% FBS as a chemoattractant ( A ). Results are presented as mean values ± SEM of the data from three separate measurements with duplicates. ** and *** indicate p < 0.01 and p < 0.001, respectively, compared to untreated control samples.

Article Snippet: The polyethylene terephthalate (PET) membrane (8 μm pore size) and the thin layer of the matrigel basement matrix in special Boyden chamber inserts (BioCoatTM Matrigel ® Invasion Chambers, Corning Inc., Corning, NY, USA) were prehydrated (2 h, serum-free EMEM) and placed onto a 24-well plate.

Techniques: Control